Magnetic Resonance service

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The NMR service is equipped with two High resolution NMR Spectrometers: a 400MHz NMR spectrometer for routine experiments and a higher filed, 600 MHz, spectrometer dedicated to high resolution magic angle spinning (HR-MAS) NMR and in vitro studies that are very demanding in terms of sensitivity. The service also has a TD-NMR analyzer for relaxivity measurements at clinical field (1.44 T).

Below we give a brief introduction to different techniques available from the Magnetic Resonance service. More detailed information can be found on the equipment pages and by contacting Dr. Carmen Muñoz, the Bionand Magnetic Resonance Specialist.

Hight Resolution Liquid NMR

    Liquid NMR is a fundamental tool both in chemistry and biomedicine research. Its applications to chemistry range from structure elucidation of small molecules to studies of ligand-protein interactions. In biomedicine, the use of liquid NMR has expanded from the classical metabolic studies using enriched stable isotope tracers to the more recent NMR-based metabolomics of biofluids (urine, plasma, CSF).

  • Bruker Avance 600 MHz NMR spectrometer
  • Bruker Ascend 400 MHz NMR spectrometer

High Resolution Magic Angle Spinning (HR-MAS) NMR

    HR-MAS NMR is based on solid-state NMR, which uses magic-angle spinning at high speeds to average anisotropic interactions, leading to narrower widths. The moderate spin rates used in HR-MAS allows high resolution spectra to be obtained from intact tissue, i.e., without the need of any metabolite extraction procedure. HR-MAS NMR is widely used for metabolomic studies, especially in cancer research, where the metabolic profile (metaboloma) of tumor biopsies can be characterized using just few milligrams of intact tissue (tissue biopsy). HR-MAS can also be used for the structure characterization and elucidation of partially insoluble compounds.

  • Bruker Avance 600 MHz NMR spectrometer + HR-MAS probe
  • HR-MAS probe (external link)

Time Domain NMR (TD-NMR)

    NMR relaxometry refers to the measurement of relaxation times, typically T1 and T2, as a function of magnetic field. This is crucial in the development of new contrast agent for MRI.

  • Bruker Minispec (TD-NMR) analyzer MQ-60